Derivation of a physical map of the chromosome of Bordetella pertussis Tohama I.
نویسندگان
چکیده
We have used pulsed-field gel electrophoresis to derive a restriction map of the chromosome of Bordetella pertussis for the enzymes XbaI, SpeI, PacI, and PmeI, which cleave 25, 16, 2, and 1 times, respectively. The apparent size of the genome is 3,750 kb. The positions of genes for major virulence determinants in the vir regulon and of some housekeeping genes were determined. Apart from the previously known linkage of the vir and fha loci, no significant linkage of virulence genes was demonstrated.
منابع مشابه
Is the Sequenced Bordetella pertussis strain Tohama I representative of the species?
Subtractive hybridization was carried out to identify differences between the sequenced genome of Bordetella pertussis Tohama I and those of two recently collected isolates. We identified genetic regions specific to recent isolates, old isolates, and isolates of B. parapertussis and B. bronchiseptica species. We conclude that Tohama I strain is not representative of the B. pertussis species.
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ورودعنوان ژورنال:
- Journal of bacteriology
دوره 174 23 شماره
صفحات -
تاریخ انتشار 1992